Hydrophilic interaction liquid chromatography (HILIC) and advanced applications

by Perry G. Wang

Publisher: Taylor & Francis in Boca Raton

Written in English
Cover of: Hydrophilic interaction liquid chromatography (HILIC) and advanced applications | Perry G. Wang
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Subjects:

  • Hydrophilic interaction liquid chromatography

Edition Notes

Includes bibliographical references and index.

Statementeditors, Perry G. Wang, Weixuan He
SeriesChromatographic science series -- v. 103
Classifications
LC ClassificationsQD79.C454 H93 2011
The Physical Object
Paginationp. cm.
ID Numbers
Open LibraryOL25077781M
ISBN 109781439807538
LC Control Number2011017684

The analysis of intact glycopeptides is a challenge because of the structural variety of the complex conjugates. In this work, we used separation involving hydrophilic interaction liquid chromatography using a superficially porous particle HALO® penta-HILIC column with tandem mass spectrometric detection for the analysis of N-glycopeptides of by: 5. A new type of core–shell composite material was prepared and applied as a hydrophilic interaction liquid chromatography (HILIC) stationary phase. In this work, silica spheres were first modified with a bimetallic central metal–organic framework (ZnCoMOF) by a new strategy of static self-assembled in situ growth. Th Analytical NanoscienceAuthor: Tiantian Si, Licheng Wang, Xiaofeng Lu, Xiaojing Liang, Shuai Wang, Yong Guo. Methods: Analysis of vortioxetine was performed in various chromatographic systems: Reversed phase (RP) systems on alkylbonded or phenyl stationary phases, hydrophilic interaction liquid chromatography (HILIC), and ion-exchange chromatography (IEC). Do you have difficulty retaining polar hydrophilic compounds using reversed phase chromatography? Our broad variety of hydrophilic interaction liquid chromatography (HILIC) columns provides strong retention of compounds that are unretained under conventional reversed phase conditions, without the requirement for ion-pair additives in the mobile phase.

  Hydrophilic interaction chromatography is a type of mixed-mode liquid chromatography, mainly used for the separation of polar compounds. When HILIC first appeared, it was considered a new type of normal phase chromatography, however, now it is thought to be much more complex than being just another type of normal phase chromatography. Reversed phase liquid chromatography (RPLC) was used in the first-dimension preparation to fractionate the sample for its high separation efficiency. After the optimization of second-dimension methods, hydrophilic interaction chromatography (HILIC) was applied to further isolate the anthocyanin for the good orthogonality to by: Alpert, A. J. () Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds. J. Chromatogr. , – PubMed CrossRef Google ScholarCited by: 4. Hernan Cortes ‐ Multidimensional chromatography: techniques and applications, M. Dekker: New York; Luigi Mondello ‐ Multidimensional chromatography, Wiley: West Sussex England; New York; Steven Cohen and Mark Schure ‐ Multidimensional liquid chromatography: theory and applications in industrial chemistry and the life sciences, Wiley‐Interscience: Hoboken, N.J;

  Hydrophilic interaction chromatography is a variation of liquid phase chromatography that overlaps in function with ion chromatography and reversed-phase liquid chromatography.   Normal phase (NP) and hydrophilic interaction liquid chromatography (HILIC) methods have shown the ability to resolve higher DP proanthocyanidins. In comparing the methods under identical conditions, HILIC shows greater retention and thus increased resolution of isomers within a given degree of polymerization [7,8]. Additionally, the HILIC Cited by: 2. In reversed-phase liquid chromatography (RPLC), positively charged basic compounds yield broad and asymmetric peaks, as a result of ionic interactions with free silanols that remain on conventional silica-based columns. Diverse solutions have been proposed to mask the silanophilic activity, which is translated to an improved peak shape. In this work, the chromatographic performance of.

Hydrophilic interaction liquid chromatography (HILIC) and advanced applications by Perry G. Wang Download PDF EPUB FB2

Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical : $ Hydrophilic Interaction Chromatography is written for scientists who use or develop analytical methods for the separation of polar compounds.

In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental samples.

Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications (Chromatographic Science Series): Medicine & Health Science Books @ Price: $ This is the first book that comprehensively and systematically describes the new technology of hydrophilic interaction liquid chromatography (HILIC).

Hydrophilic interaction chromatography is a separation technique suitable for polar and hydrophilic compounds and orthogonal to reversed phase liquid cturer: CRC Press.

Book Description. This is the first book that comprehensively and systematically describes the new technology of hydrophilic interaction liquid chromatography (HILIC). Hydrophilic interaction chromatography is a separation technique suitable for polar and hydrophilic compounds and orthogonal to reversed phase liquid chromatography.

Hydrophilic Interaction Chromatography is written for scientists who use or develop analytical methods for the separation of polar compounds. In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental cturer: Wiley.

Hydrophilic interaction liquid chromatography (HILIC) has become in the last years a leading technique for the separation of small and large polar molecules. This chapter begins with a discussion about the most relevant characteristics of HILIC mode that makes it a complementary and, in some cases, alternative technique to the more traditional.

Comprehensive Guide to HILIC: Hydrophilic Interaction Chromatography (Waters Series) 1st Edition by Waters Corporation (Author) ISBN ISBN Why is ISBN important. ISBN. This bar-code number lets you verify that you're getting exactly the right version or edition of a book.

Format: Paperback. Book Review: Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications Gabriel Marcelín Jiménez Global Bioanalytical Consulting, S.C., Av. Hydrophilic interaction liquid chromatography book CuauhtémocOfna.Col. Piedad-Narvarte, Mexico City, c.p.

: Gabriel Marcelín Jiménez. Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods Percoll Methodology and Applications Ficoll-Paque Plus For in vitro isolation of lymphocytes GST Gene Fusion System Handbook Antibody Purification Handbook File Size: 2MB.

in their book Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications, published by CRC Press (Taylor & Francis Group, Boca Raton, FL, USA, ). This is a well-organized piece that takes us through 23 chapters ranging from the most basic concepts up to the very specific applica-tions developed for recognized scientific teams who make contributions to the book.

Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical applications.

Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography.

HILIC uses hydrophilic stationary phases with reversed-phase type eluents. A novel hydrophilic interaction liquid chromatography method for the analysis of arterolane; Isolation and structural elucidation of its major degradation product by LC-HRMS and NMR Author links open overlay panel Prakash Niguram a Ashutosh Goswami b Abhijeet S.

Kate a b. In hydrophobic interaction liquid chromatography, the degree of MI is obtained as the change in VW energy values. Simple molecules can be used to demonstrate the major MI mechanisms [31a]; however, an ideal model phase is required for a practical contact File Size: KB.

Jump into the HPLC adventure. Three decades on from publication of the 1 st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the. Hydrophilic interaction liquid chromatography (HILIC) has become in the last years a leading technique for the separation of small and large polar molecules.

This chapter begins with a discussion about the most relevant characteristics of this chromatographic mode that makes HILIC a complementary and, in some cases, alternative technique to the more traditional reversed- and normal-phase by: 7.

Today, the most rational way to address the separation of very hydrophilic and uncharged compounds in HPLC is by utilizing hydrophilic interaction liquid chromatography (HILIC), which is considered to be a variant of normal‐phase chromatography.

Hydrophilic interaction liquid chromatography (HILIC) is suitable for separations of strongly or moderately polar samples on polar columns in aqueous–organic mixed. A novel chitooligosaccharide stationary phase for hydrophilic interaction liquid chromatography (HILIC) was developed via click chemistry and showed great HILIC characteristics on separation of polar compounds and enrichment of glycopeptides.

Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development.

This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical : Wiley.

Offering a comprehensive review, this book enables readers to develop a fundamental understanding of how HILIC works and then apply that knowledge to develop and implement a variety of practical applications."Hydrophilic Interaction Chromatography" begins with discussions of HILIC retention mechanisms, stationary phases, and general method development.

ISBN: OCLC Number: Description: xx, pages: illustrations ; 24 cm. Contents: Aqueous normal-phase chromatography: the bridge between reversed-phase and HILIC / Joseph J.

Pesek, Maria T. Matyska --Application of the calculated physicochemical property, log D, to hydrophilic interaction chromatography in the bioanalytical laboratory / Eugene.

Abstract. In contrast to achiral hydrophilic interaction liquid chromatography (HILIC), which is a popular and largely applied technique to analyze polar compounds such as pharmaceuticals, metabolites, proteins, peptides, amino acids, oligonucleotides, and carbohydrates, the introduction of the HILIC concept in enantioselective chromatography has been relatively recent and scarcely : Roberto Cirilli.

The Comprehensive Guide to HILIC: Hydrophilic Interaction Chromatography, a page book, illustrates how HILIC works and how separation scientists can improve their success in separating and quantifying polar compounds in a variety of sample matrices.

hydrophilic interaction liquid chromatography (HILIC), focusing on the retention mechanisms of eleven stationary) users during HILIC method development.

Hydrophilic Interaction Liquid Chromatography: Method Development Approaches Thermo Fisher Scientific, Runcorn, Cheshire, UK). •File Size: 1MB. The Comprehensive Guide to HILIC: Hydrophilic Interaction Chromatography, a page book, illustrates how HILIC works and how separation scientists can improve their success in separating and quantifying polar compounds in a variety of sample matrices.

This paper covers fundamental developments in hydrophilic interaction liquid chromatography. The objective of the present work is to review options for the characterization of HILIC stationary phases and their applications to separations of polar compounds in complex by: Hydrophilic interaction liquid chromatography (HILIC) is a complementary technique to both normal and reversed-phase chromatography.

It employs polar stationary phases (bare silica, aminopropyl, amide, diol, or zwitterionic phases) and organic-rich, low aqueous mobile phases to elute the analytes in order of increasing polarity.

Hydrophilic Interaction Liquid Chromatography. Volume 1. Xinmiao Liang. Key Laboratory of Separation, Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P.R., China. Search for more papers by this author. Aijin : Xinmiao Liang, Aijin Shen, Zhimou Guo.

Hydrophilic interaction chromatography (HILIC) is a rapidly developing liquid chromatography mode suitable for separation of strongly or moderately polar samples on polar columns in highly organic.Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field.With authentic 2-methyltetrol and methyltetrol sulfate standards synthesized in-house, we developed a hydrophilic interaction liquid chromatography (HILIC)/ESI-HR-quadrupole time-of-flight mass spectrometry (QTOFMS) protocol that can chromatographically resolve and accurately measure the major IEPOX-derived SOA constituents in both laboratory.